Document Type : Original Article
Authors
1
Suez Canal University, Faculty of Agriculture, Plant Protection Department, Ismailia-41522, Egypt
2
Plant Protection Institute, ARC, Ismailia, Egypt
3
Laboratoire d’Eco-Toxicologie, UPRES-EA 2069 Faculte´ des Sciences, Universite´ de Reims Champagne-Ardenne, B.P. 1039, 51687 Reims cedex 02, France
10.21608/jpces.2008.459600
Abstract
Laboratory studies were conducted to determine the effects of different concentrations of pyrimethinal (5, 10, 15, 20 and 25 mg/l) on protein content, and some oxidative stress parameters in an aquatic worm, Tubifex tubifex after 2, 4, and 7 days of exposure. In addition, residues of the fungicide were quantified in water and in the worms. In water, pyrimethinal concentration decreased slowly (maximum – 6.4 ± 0.8% after 2 days for 25 mg/l). In the worms, it increased after 4 days and decreased thereafter, confirming that the worms were exposed to the fungicide and not to a degradation product. LC50 values of pyrimethinal were between 49.2 ± 0.58 and 39.5 ± 0.95 mg/l depending on exposure time. All the measured parameters appeared to be more sensitive than mortality. The activity of catalase (CAT) increased in response to the fungicide after 2 days of exposure to 25 mg/l of pyrimethinal (+ 84.95 %), demonstrating an oxidative stress in the worms. In contrast GST activity decreased, this decrease was significant for all fungicide concentrations after two days. The highest decrease of GST activity (- 29.7 %) was found after 7 days in the presence of 25 mg/l of pyrimethinal .
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