Genotoxic effects of three different tested pesticides coumatetralyl, ivermectin and imidacloprid at sublethal dose on albino rats Rattus norvegicus albinus

Document Type : Original Article

Authors

1 Etay El-Baroud Agric. Research Station, A.R.C.

2 Faculty of Agric. Menofia University

10.21608/jpces.2009.459621

Abstract

Three pesticides from three different chemical groups; coumatetraryl, imidacloprid and ivermectin were used at sublethal doses of 0.1 and 0.25 LD₅₀ to perform the genotoxic effects on albino rats Rattus norvegicus albinus by determining the total protein and nucleic acids (RNA and DNA) in brain, kidney, liver, heart and spleen. Coumatetraryl (0.1 LD₅₀) caused significant increase in DNA quantity in all tested tissues except in spleen. Whereas 0.25 LD₅₀ caused highly significant increase in DNA quantity in brain and heart tissues and significant only in spleen. There were parallel results concerning RNA quantities with DNA obtained data especially upon using 0.25 LD₅₀ coumatetraryl caused genotoxic effect in treated rats. This was pronounced in highly significant values for protein content that was obtained upon treatment by 0.1 and 0.25 LD₅₀ in most compounds tested. The imidacloprid caused significant increase in DNA content in all tested tissues, except in the heart where it showed highly significant increase over the control value. Regarding RNA content estimated in various tissues upon imidaclopid treatment, it showed severe reduction at both used doses, being significant in the liver, and highly significant in spleen and kidney. Total protein level showed highly significant decrease in all tested tissues under the two used concentrations. Ivermectin exerted its genotoxic effect on liver tissue, as it caused highly significant increase in DNA content under both concentrations. Whereas, RNA content showed highly significant changes being positive (increase) in heart tissue and negative (decrease) in kidney and spleen tissues upon using 0.1 LD₅₀ while 0.25 LD₅₀ caused highly significant decrease of RNA value in all tested tissues except liver tissue, where it showed highly significant increase in quantity.
Journal of Pest Control and Environmental Sciences
Volume 9, Issue 3
December 2009
Pages 13-26

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